Application

Detect the Glutathione: N-ethylmaleimide adduct protein using this Anti-Glutathione: N-ethylmaleimide adduct, clone 8.1GSH validated for use in ELISA, Flow, ICC, IHC & WB.

Immunohistochemistry: 1:100 (paraformaldehyde fixation)

Immunocytochemistry: 1:100 - 1:500 for assessment of intracellular GSH distribution.

ELISA: 1:100,000 - 1:200,000 to quantify GSH at >2 mM in biological specimens.

Flow cytometry: 1:100 - 1:1,000

Cells were fixed with cold 1% PFA for 5 min and vortexed into methanol (-20°C) containing 5mM NEM (10 min on dry ice) or 10 µM NEM (N-ethyl maleimide) for 30 min at RT.

Optimal working dilutions must be determined by end user.

Positive Control: Peripheral blood mononuclear cells (PBMC) treated with NEM

Research CategoryNeuroscience

Research Sub CategoryOxidative Stress

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

GS-NEM conjugated to KLH

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Liquid in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

Format: Purified

Specificity

Reactive with thiol-modified glutatione. Does not react with oxidized glutathione (GSSG) or glutathione (GSH) in mixed disulfide linkage with proteins. MAB3194 is specific to the glutathione:N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide. For use in ELISA or flow cytometry, sample must first be reacted with NEM.

Storage and Stability

Maintain at 2-8°C in undiluted.